18O-Studies with Citrate Synthase and Malate Synthase
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چکیده
منابع مشابه
Interaction between Citrate Synthase and Malate Dehydrogenase
The interactions between pig heart citrate synthase and mitochondrial malate dehydrogenase or cytosolic malate dehydrogenase were studied using the frontal analysis method of gel filtration and by precipitation in polyethylene glycol. This method showed that an interaction between citrate synthase and mitochondrial malate dehydrogenase occurred but no interaction between citrate synthase and cy...
متن کاملNoninvolvement of acyl carrier protein with citrate synthase and malate synthase.
Acyl carrier protein (ACP coli) was isolated from commercially grown Escherichia coli B and was acetylated by chemical methods. Biological activity of the synthesized acetyl-ACP coli was checked in an in vitro fatty acid-synthesizing system isolated from E. coli B. Since acetyl-ACP is preferred over acetyl-coenzyme A (CoA) as a substrate in these reactions, the possibility that it may substitut...
متن کاملQuantitation of the interaction between citrate synthase and malate dehydrogenase.
Formation of a bienzyme complex of pig heart mitochondrial malate dehydrogenase and citrate synthase in a buffered system is demonstrated by means of a covalently attached fluorescent probe to citrate synthase. Assuming 1:1 stoichiometry of the enzymes in the complex, an apparent dissociation constant of 10(-6) M was calculated from fluorescence anisotropy measurements. The effect of various me...
متن کاملBiochemical and structural studies of malate synthase from Mycobacterium tuberculosis.
Establishment or maintenance of a persistent infection by Mycobacterium tuberculosis requires the glyoxylate pathway. This is a bypass of the tricarboxylic acid cycle in which isocitrate lyase and malate synthase (GlcB) catalyze the net incorporation of carbon during growth of microorganisms on acetate or fatty acids as the primary carbon source. The glcB gene from M. tuberculosis, which encode...
متن کاملInteraction between citrate synthase and malate dehydrogenase. Substrate channeling of oxaloacetate.
The interactions between pig heart citrate synthase and mitochondrial malate dehydrogenase or cytosolic malate dehydrogenase were studied using the frontal analysis method of gel filtration and by precipitation in polyethylene glycol. This method showed that an interaction between citrate synthase and mitochondrial malate dehydrogenase occurred but no interaction between citrate synthase and cy...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1969
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1969.tb00745.x